Composite

Part:BBa_K4139022:Design

Designed by: Zachary Robinson & Jason Luddu   Group: iGEM22_Lethbridge   (2023-09-27)


Full Length PbEL04


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 998
    Illegal PstI site found at 1124
    Illegal PstI site found at 1247
    Illegal PstI site found at 1370
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal PstI site found at 998
    Illegal PstI site found at 1124
    Illegal PstI site found at 1247
    Illegal PstI site found at 1370
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 998
    Illegal PstI site found at 1124
    Illegal PstI site found at 1247
    Illegal PstI site found at 1370
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 998
    Illegal PstI site found at 1124
    Illegal PstI site found at 1247
    Illegal PstI site found at 1370
    Illegal AgeI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted a full length version of PbEL04 so that we can test our antibody with the proteins seen in field. We added a His-tag at the end to assist with purification.

Source

Plasmodiophora brassicae

References

Jiang, X., Su, Y., & Wang, M. (2022). A small cysteine-rich protein identified from the Proteome of clubroot pathogen, Plasmodiophora brassicae, induces cell death in nonhost plants and host plants. https://doi.org/10.21203/rs.3.rs-1961445/v1